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ÀÌȫǥ (Hong-pyo Lee) ÀúÀÚ À̸ÞÀÏ º¸±â
Stanford University
 
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Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments
¿­±â Authors and Affiliations

Abstract

For mesenchymal stem cells (MSCs) cultured in three dimensional matrices, matrix remodeling is associated with enhanced osteogenic differentiation. However, the mechanism linking matrix remodeling in 3D to osteogenesis of MSCs remains unclear. Here, we find that MSCs in viscoelastic hydrogels exhibit volume expansion during cell spreading, and greater volume expansion is associated with enhanced osteogenesis. Restriction of expansion by either hydrogels with slow stress relaxation or increased osmotic pressure diminishes osteogenesis, independent of cell morphology. Conversely, induced expansion by hypoosmotic pressure accelerates osteogenesis. Volume expansion is mediated by activation of TRPV4 ion channels, and reciprocal feedback between TRPV4 activation and volume expansion controls nuclear localization of RUNX2, but not YAP, to promote osteogenesis. This work demonstrates the role of cell volume in regulating cell fate in 3D culture, and identifies TRPV4 as a molecular sensor of matrix viscoelasticity that regulates osteogenic differentiation.

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- Çü½Ä: Research article
- °ÔÀçÀÏ: 2019³â 01¿ù (BRIC µî·ÏÀÏ 2019-02-07)
- ¿¬±¸Áø: ±¹¿Ü¿¬±¸Áø
RNF20/40 ÀÇÁ¸Àû eEF1B¥äL ¸ð³ëÀ¯ºñÄûƾȭ¿¡ ÀÇÇÑ ¿­Ãæ°Ý À¯ÀüÀÚ Àü»ç Á¶Àý[Nucleic Acids Res.]
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Google (by Hong-pyo Lee)
Pubmed (by Hong-pyo Lee)
ÇÁ¸®¹Ì¾ö BioÀÏÁ¤ BioÀÏÁ¤ ÇÁ¸®¹Ì¾ö ¾È³»
2019 Korean Researchers¡¯ Night in AACR Âü°¡ ½Åû ¾È³» (¹Ì±¹ ¾ÖƲ·£Å¸)
2019 Korean Researchers¡¯ Night in AACR Âü°¡ ½Åû ¾È³» (¹Ì±¹ ¾ÖƲ·£Å¸)
»çÀüÁ¢¼ö: ~2019.02.20
³¯Â¥: 2019.03.31
Àå¼Ò: Atlanta Marriott Marquis
[BRIC Webinar]RNF20/40 ÀÇÁ¸Àû eEF1B¥äL ¸ð³ëÀ¯ºñÄûƾȭ¿¡ ÀÇÇÑ ¿­Ãæ°Ý À¯ÀüÀÚ Àü»ç Á¶Àý-Àμ±¾Æ (KAIST)
´ëÇѰñ´ë»çÇÐȸ The 7th Seoul Symposium on Bone Health & Á¦31Â÷ Ãá°èÇмú´ëȸ
´ëÇѰñ´ë»çÇÐȸ The 7th Seoul Symposium on Bone Health & Á¦31Â÷ Ãá°èÇмú´ëȸ
ÃÊ·ÏÁ¢¼ö: ~2019.02.28
»çÀüÁ¢¼ö: ~2019.04.01
³¯Â¥: 2019.05.30~06.01
Àå¼Ò: ¼­¿ïµå·¡°ï½ÃƼ 5Ãþ ¹éµÎ·ë
2019 ¼­°æ¹è°úÇÐÀç´Ü ½ÅÁø°úÇÐÀÚ ¿¬±¸Áö¿ø ÇÁ·Î±×·¥ °ø°í
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