[DEBUG-WINDOW 처리영역 보기]
즐겨찾기  |  뉴스레터  |  오늘의 정보 회원가입   로그인
BRIC홈 농수식품
웨비나 모집
스폰서배너광고 안내  배너1
전체보기 농림성과 식품성과 인터뷰
사진없음
이제희 (Jehee Lee) 저자 이메일 보기
제주대학교
조회 86  인쇄하기 주소복사 트위터 공유 페이스북 공유 
Two phospholipid scramblase 1–related proteins (PLSCR1like-a & -b) from Liza haematocheila: Molecular and transcriptional features and expression analysis after immune stimulation

W.M.Gayashani Sandamalika1, Thanthrige Thiunuwan Priyathilaka1, and Bo-Hye Nam2, and Jehee Lee1*

a
Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea
b Biotechnology Research Division, National Institute of Fisheries Science, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan, 46083, Republic of Korea

*Corresponding author : Jehee Lee, Marine Molecular Genetics Lab, Department of Marine Life Sciences, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea.

Abstract
Phospholipid scramblases (PLSCRs) are a family of transmembrane proteins known to be responsible for Ca2+-mediated bidirectional phospholipid translocation in the plasma membrane. Apart from the scrambling activity of PLSCRs, recent studies revealed their diverse other roles, including antiviral defense, tumorigenesis, protein-DNA interactions, apoptosis regulation, and cell activation. Nonetheless, the biological and transcriptional functions of PLSCRs in fish have not been discovered to date. Therefore, in this study, two new members related to the PLSCR1 family were identified in the red lip mullet (Liza haematocheila) as MuPLSCR1like-a and MuPLSCR1like-b, and their characteristics were studied at molecular and transcriptional levels. Sequence analysis revealed that MuPLSCR1like-a and MuPLSCR1like-b are composed of 245 and 228 amino acid residues (aa) with the predicted molecular weights of 27.82 and 25.74?kDa, respectively. A constructed phylogenetic tree showed that MuPLSCR1like-a and MuPLSCR1like-b are clustered together with other known PLSCR1 and -2 orthologues, thus pointing to the relatedness to both PLSCR1 and PLSCR2 families. Two-dimensional (2D) and 3D graphical representations illustrated the well-known 12-stranded β-barrel structure of MuPLSCR1like-a and MuPLSCR1like-b with transmembrane orientation toward the phospholipid bilayer. In analysis of tissue-specific expression, the highest expression of MuPLSCR1like-a was observed in the intestine, whereas MuPLSCR1like-b was highly expressed in the brain, indicating isoform specificity. Of note, we found that the transcription of MuPLSCR1like-a and MuPLSCR1like-b was significantly upregulated when the fish were stimulated with poly(I:C), suggesting that such immune responses target viral infections. Overall, this study provides the first experimental insight into the characteristics and immune-system relevance of PLSCR1-related genes in red lip mullets.

Key words: Phospholipid scramblase 1 like, PLSCR, Red lip mullet, Liza haematocheila, immune response

논문정보
- 형식: Research article
- 게재일: 2018년 12월 (BRIC 등록일 2019-01-02)
- 연구진:
- 분야: Immunology, Marine_Biology, Veterinary_Science
  댓글 0
등록
 
목록
바이오코리아
외부관련링크
Google scholar (by Jehee Lee)
Pubmed (by Jehee Lee)
프리미엄 Bio일정 Bio일정 프리미엄 안내
[BRIC Webinar]성공적인 CRISPR-Cas9 유전자 편집 실험을 위한 최신기술 소개-장윤희 (Thermo Fisher Scientific)
2019 서경배과학재단 신진과학자 연구지원 프로그램 공고
[BRIC Webinar]생존 운동 신경세포에서의 circular RNA 생성-서준배 (Iowa State University)
이매스교육센터 3월, 4월 교육일정 안내
이매스교육센터 3월, 4월 교육일정 안내
사전접수: ~2019.04.17
날짜: 2019.04.18~19
장소: 구로디지털단지 우림이비지센터2차 1406호 (2호선 구로디지털단지역 3번출구 도보 10분)
국제연구인력교류사업 (해외고급과학자 초빙사업, 해외우수신진연구자 유치사업) 공모
CJ제일제당 BLOSSOM IDEA LAB 1기 모집
CJ제일제당 BLOSSOM IDEA LAB 1기 모집
날짜: 2019.03.31
주관: CJ제일제당
 
위로가기
농수식품 홈  |  농수식품 문의 및 제안
 |  BRIC소개  |  이용안내  |  이용약관  |  개인정보처리방침  |  이메일무단수집거부
Copyright © BRIC. All rights reserved.  |  문의 member@ibric.org
트위터 트위터    페이스북 페이스북    RSS서비스 RSS
다윈바이오
1553127417 0.12350600
1553127418 0.32887300
1.2053668498993 초 소요